454 pyrosequencing v1–v3 regions,16srrna  (Pyrosequencing Inc)

Journal: Journal of Dental Sciences

Article Title: Oral microbial profiles of extrinsic black tooth stain in primary dentition: A literature review

doi: 10.1016/j.jds.2024.02.028

Figure Lengend Snippet: Oral microbial profiles of the EBS in primary dentition.

Article Snippet: 2016 , China , 40 EBS; 26 HG , saliva plaque EBS , 454 pyrosequencing (V1–V3 regions,16SrRNA) , Relative abundance: • Plaque-EG vs. HG: Leptotrichia and Fusobacterium ( P < 0.05–0.001, EG↑) • Saliva-No significant difference.

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Human Male Genital Tract Microbiota

doi: 10.3390/ijms24086939

Figure Lengend Snippet: Characterisation of penile microbial communities using NGS.

Article Snippet: Nelson et al., 2012 [ ] , Pyrosequencing (V1–V3, V3–V5 and V6–V9) , 18 , , No , Characterisation of coronal sulcus (CS) and urine microbiota in adolescent men. The three most abundant genera were Corynebacterium , Staphylococcus , and Anaerococcus , representing more than 58.9% of total sequences, followed by Peptoniphilus , Prevotella , Finegoldia , Porphyromonas , Propionibacterium, and Delftia . CS microbiota was stable during study interval, especially for Staphyloccoccus , Mobiluncus , Prevotella , Dialister, and Anaerococcus genera. Distinct bacterial communities between CS and urine samples and between circumcised and noncircumcised men..

Techniques: Sequencing, Comparison, Bacteria, Virus

Journal: International Journal of Molecular Sciences

Article Title: Human Male Genital Tract Microbiota

doi: 10.3390/ijms24086939

Figure Lengend Snippet: Characterisation of seminal microbiota using NGS.

Article Snippet: Nelson et al., 2012 [ ] , Pyrosequencing (V1–V3, V3–V5 and V6–V9) , 18 , , No , Characterisation of coronal sulcus (CS) and urine microbiota in adolescent men. The three most abundant genera were Corynebacterium , Staphylococcus , and Anaerococcus , representing more than 58.9% of total sequences, followed by Peptoniphilus , Prevotella , Finegoldia , Porphyromonas , Propionibacterium, and Delftia . CS microbiota was stable during study interval, especially for Staphyloccoccus , Mobiluncus , Prevotella , Dialister, and Anaerococcus genera. Distinct bacterial communities between CS and urine samples and between circumcised and noncircumcised men..

Techniques: Sequencing, Bacteria, Infection, Concentration Assay, Virus, Isolation

Journal: International Journal of Molecular Sciences

Article Title: Molecular Accounting and Profiling of Human Respiratory Microbial Communities: Toward Precision Medicine by Targeting the Respiratory Microbiome for Disease Diagnosis and Treatment

doi: 10.3390/ijms24044086

Figure Lengend Snippet: Summary of the published studies related to the respiratory microbiome in COPD patients.

Article Snippet: 2011 , Healthy smokers, nonsmokers, and COPD subjects, USA. , 4 , To explore the differences in the lung microbiome of the three groups. , 16S rRNA pyrosequencing (v1-V3 region). Taxonomic and phylogenetic-based analysis l6s rDNA. The qPCR was used to quantify the total bacterial load. , Healthy smokers, nonsmokers, and mild COPD tend to have a more diverse microbial community than moderate and severe COPD microbiota. There was no significant difference in the microbiota and the bacterial load between the three study groups. A core microbiota was identified and includes: “ Pseudomonas , Streptococcus , Prevotella , Fusobacterium , Haemophillus , Veillonella ”. Significant microanatomic changes in bacterial population were observed within the same lung of advanced COPD patients. , [ ] .

Techniques: Microarray, Biomarker Discovery, Control, Bacteria, Sequencing, Sterility, Positive Control, Cloning

Journal: Gut Microbes

Article Title: The double-edged sword of probiotic supplementation on gut microbiota structure in Helicobacter pylori management

doi: 10.1080/19490976.2022.2108655

Figure Lengend Snippet: Summary of studies examining the effects of probiotic co-supplementation to H. pylori eradication on the human gut microbiota.

Article Snippet: Oh et al., 2016 , 10 subjects in each group , Amoxicillin 1 g bid, clarithromycin 500 mg bid, lansoprazole 30 mg bid, 14 days , Streptococcus faecium 9 × 10 8 , Bacillus subtilis 1 × 10 8 , twice daily, 14 days , 16S rRNA gene-pyrosequencing (V1-V3) , 2 weeks , Citrobacter, Klebsiella, Pseudomonas , and Escherichia , NS.

Techniques: Sequencing, Capsules, Maltodextrin

Journal: Frontiers in Oral Health

Article Title: Meta-Analysis Using NGS Data: The Veillonella Species in Dental Caries

doi: 10.3389/froh.2021.770917

Figure Lengend Snippet: Methodological characteristics of the included studies ( n = 38).

Article Snippet: Simon-Soro et al. (2013) , Spain , [ ] , ND , NA , Nyvad 2003 , Active enamel caries; cavitated dentinal caries; hidden dentinal caries , 1 , V1–V3, 454 pyrosequencing , +.

Techniques: Control, Sequencing, Activity Assay

Journal: Microorganisms

Article Title: Improvement of Soil Microbial Diversity through Sustainable Agricultural Practices and Its Evaluation by -Omics Approaches: A Perspective for the Environment, Food Quality and Human Safety

doi: 10.3390/microorganisms9071400

Figure Lengend Snippet: -Omics approaches applied to soil microbial diversity studies.

Article Snippet: Bacteria and fungi , high-throughput pyrosequencing of bacterial 16S V1-V3 and fungal ITS 2 of the ribosomal DNA operon , Organic farming increases microbial soil microbial diversity , [ ] .

Techniques: Bacteria, Next-Generation Sequencing, Amplification, Sequencing, Activity Assay